![]() ![]() Pruning plants to allow for good air circulation and reducing crowding will also help keep the foliage dry.Ĥ. Watering can also spread the disease by splashing. Use soaker hoses or water early in the day so the foliage can dry off before night. This is not a cure but may help limit infection by reducing the total amount of inoculum.ģ. Raking up and disposing of infected leaves as they drop and pruning out dead twigs can help control the disease by removing spores that can reinfect the new leaves. Only if defoliation occurs three or more years in a row will most established plants be adversely affected.Ģ. A tree affected early in the year will re-leaf and the new leaves may not be affected. Most trees tolerate leaf spots with little or no apparent damage. Overhead watering can also provide prolonged wet periods that are ideal for spreading leaf spot diseases.ġ. ![]() During wet weather, spores may splash or be windblown onto newly emerging tender leaves where they germinate in the moisture and infect the leaf. Some may remain in dead twigs on the tree. The organisms that cause leaf spots survive in fallen infected leaves and twigs. Spots or blotches that are angular are generally referred to as anthracnose (see entry on “Anthracnose of Trees”) Leaves may yellow and drop prematurely. Over time, the spots may combine or enlarge to form blotches. Fungal bodies may appear as black dots in the spots, either in rings or in a central cluster. Concentric rings or dark margins are often present. ![]() Spots are most often brownish, but may be tan or black. The spots will vary in size and color depending on the plant affected, the specific organism involved, and the stage of development. The chief symptom of a leaf spot disease is spots on foliage. Small or newly planted trees that become defoliated are more at risk of suffering damage until they become established. An established plant can tolerate almost complete defoliation if it happens late in the season or not every year. Leaf spot may result in some defoliation of a plant. Leaf spots on trees are very common and generally do not require spraying. Some insects also cause damage that appears like a leaf spot disease. The majority of leaf spots are caused by fungi, but some are caused by bacteria. Leaf spot is a common descriptive term applied to a number of diseases affecting the foliage of ornamentals and shade trees. Close-up of fungal tar spot on sugar maple ( Acer saccharum) note the characteristic ripples or wavy indentations in the black, tarry surface of the lesion ![]()
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![]() Before the end of the second quarter 2015, Tidal is expected to launch in Poland, Australia, Hong Kong, Quatar, United Arab Emirates and Germany. Tidal users can stream music on iOS and Android mobile phones, tablets, or desktops in 31 countries including: US, Canada, the UK, Ireland, Finland, the Netherlands, Belgium, Luxembourg, Italy, South Africa, Czech Republic, Slovakia, Singapore, Denmark, Spain, Portugal, Estonia, Latvia, Lithuania, Norway, France, Austria, Switzerland, Hungary, Turkey, Sweden, Romania, Slovenia, Greece, Cyprus and Malta. Users can try Premium for free for 30 days. For $9.99, Spotify Premium gives all the above plus offline mode on the user's computer and mobile, exclusive content, enhanced sound quality and the ability to play through multi-room music systems.You can also play any song, anytime without ads. Spotify Unlimited will cost a user $4.99 a month, but along with access to instant streaming, users can also use Spotify Radio mode, take music abroad, with no time limits. The free subscription allows users access to millions of tracks for instant streaming on your smartphone, tablet, or computer, make new playlists, and browse playlists based on moods and genres. Spotify offers its users three service options: Spotify Free, Spotify Unlimited, Spotify Premium. However, users can get a free seven-day trial. That means those who opt for the $19.99 per month Tidal subscription will be paying twice as much as Spotify users. Tidal does not offer a free subscription option for its users, making its standard subscription as much as Spotify Premium. Same exclusive content, same access to the music & video #TIDALforALL ![]() and the UK last year, charging music lovers either $9.99 for standard quality audio, or $19.99 a month to stream high-fidelity16-bit FLAC digital audio files.Īccording to its Twitter account, Tidal says it will offer a new Premium subscription option for $9.99 that will include the same access to music and video, whereas the more expensive subsciption includes CD-quality streaming.īrand spankin' new- $9.99 Premium subscription. Tidal, the new rival to Spotify, launched in both the U.S. By using a smartphones or tablets as a remote to control, gamers can change songs without pausing their game or switching apps. ![]() PS4 users will now be able to play games while listening to music at the same time directly from their consoles. Spotify made headlines today thanks to Sony's launch of the music streaming service on its PS3 and PS4 game consoles in 41 countries. The content creator should be compensated. ![]() "Music is everywhere -you consume it every day, everywhere you go. "Everywhere else, everyone gets compensated for their work," he said. Jay Z emphasized that music streaming has been unfair for artists in terms of compensation. The platform may also turn into a hub of entertainment with the possibility to sell concert ticks and merchandise. "This is a platform that's owned by artists," Jay Z said. "We are treating these people that really care about the music with the utmost respect." ![]() ![]() ![]() You can have peace of mind knowing our professionals will handle everything from the initial planning to construction. We have an entire team dedicated to constructing homes under this exact circumstance. While we typically help families find the plot of land they want to build their luxury home on, we can also build on your existing lot. You can enjoy peaceful living away from the hectic city life Build on Your Own Lot in Shawneeĭo you have an existing lot in Shawnee you want to build on? The team of experts at Richardson homes can help you and your family build on your own lot. ![]() Rural settings are typically a just short drive from shopping centers and entertainment.The price of property outside Metro OKC is lower.There are no limitations due to additional costs and zoning restrictions.You can build your dream home for the price you want.If you are a human, do not fill in this field. The rural home builders at Richardson Homes understand all the benefits families can experience with a custom-built home in a rural setting, including: Call us today to learn more! Beautiful Rural Custom Homes in Shawneeĭo you dream of owning a luxury custom home with a spectacular view of the countryside? Some families prefer to thrive in less populated areas of the state, including Shawnee. Specializing in rural and city builds throughout Shawnee, OK, and surrounding areas, our team has decades of combined experience in designing and constructing luxury homes that are made to last. Our highly experienced team of custom home designers will guide you through each step in the process, from design to construction. Whether you want a custom home built in a rural setting in Shawnee or closer to the town’s center, our homes are tailored to fit all your family’s needs. If you’re in the market for a new home in Shawnee, OK and the surrounding areas, the luxury home designers at Richardson Homes have been helping residents bring their homeownership dreams to life for nearly 25 years. It does not store any personal data.Looking for Luxury Custom Home Designers Near Shawnee, OK? Let Our Custom Home Builders Design the House of Your Dreams The cookie is set by the GDPR Cookie Consent plugin and is used to store whether or not user has consented to the use of cookies. The cookie is used to store the user consent for the cookies in the category "Performance". This cookie is set by GDPR Cookie Consent plugin. The cookie is used to store the user consent for the cookies in the category "Other. ![]() The cookies is used to store the user consent for the cookies in the category "Necessary". The cookie is set by GDPR cookie consent to record the user consent for the cookies in the category "Functional". The cookie is used to store the user consent for the cookies in the category "Analytics". These cookies ensure basic functionalities and security features of the website, anonymously. Necessary cookies are absolutely essential for the website to function properly. ![]() ![]() Barney can be your friend too, If you just make-believe him! Trivia ĭoll 4.pngBarney has his Season 3 voice and 1994-1997 costume. Barney comes to play with us Whenever we may need him. After school they meet to play And sing with happy faces Barney shows us lots of things Like how to play pretend, ABC's and 123s And how to be a friend. Barney can be your friend too, If you just make-believe him! 2004-2009 Version Barney is a dinosaur From our imagination, And when he's tall, he's what we call A dinosaur sensation! Barney's friends are big and small They come from lots of places. Scenes from the Theme Song with lyrics of the Theme Song in place are: Original 1996 version Barney is a dinosaur from our imagination And when he's tall, he's what we call a dinosaur sensation Barney lives with Michael and With Amy, then you'll soon see Little Tina tags along With her big sister Luci Derek is in Barney's club And Jeffrey has a ticket But Mom has never seen him, 'Cause she doesn't know our secret Barney comes to play with us Whenever we may need him Barney can be your friend too If you just make-believe him! 1997-1999 Version Barney is a dinosaur From our imagination, And when he's tall, he's what we call A dinosaur sensation! Barney's friends are big and small They come from lots of places. Hello, Goodbye (Bob Singleton Musical Arrangements Vocals Director) (Season 3 Home Video Version) (Scene Taken from: Shopping for a Surprise and Barney's Talent Show).Respect! (Bob Singleton Musical Arrangements Vocals Director) (Season 3 Home Video Version) (Scene Taken from: Barney's Talent Show) (Production for This Video Was Filmed Began in November 1-30,.Baby Bop (Jennifer Romano as body performer, Julie Johnson as voice).(Kyle Nelson as body performer, Patty Wirtz as voice) Barney (Carey Stinson as body performer, Bob West as voice).10.6 Decem(Hit Entertainment reprint release)īarney, Baby Bop, BJ and the kids learn how to be good friends to others and respecting them.10.5 April 14th 2001 (Anchor Bay Entertainment rarity version).10.4 Decem(Paramount/Nickelodeon release).10.3 April 17th, 1999 (Lyrick Studios Reprint release).10.2 Novem(Lyrick Studios Printing release).10.1 Original Octo(PBS Home Video Printing release).10 Barney's Let's Show Respect Previews.4 Scenes from the Theme Song with lyrics of the Theme Song in place are:.22 Barney: Camp Wannarunnaround Credits Comparison (Screener vs.21 Barney: Camp WannaRunnaRound (All Versions) & 2018S & 2018 (Play for Exercise!).19 Happy Valentine’s Day! (Be My Valentine, Love, Barney) (I Love You (Hearts) (I Love You Too!!!!).18 Hannah & Kim From: It's Home to Me! & Let's Build Together.17 It's Time for Counting (All Week of All That For Screener & Original).15 It's Showtime! & Good Job! (Treehouse Aired In December 16, 2004) & WNED.14 Barney's Talent Show (Screener) & Twice Is Nice! (Mostly Complete Episode). ![]() ![]() ![]() ![]() 13 Play and Learn with Barney (Good Manners).12 Barney: Camp Wannarunnaround Credits Comparison (Screener vs.11 Barney: It's Time for Counting (All Versions).10 Barney in Concert Opening & Closing For 1995 VHS.9 Barney's Let's Go to the Zoo (All Versions) (Demo Version) is Coming Soon.7 Time for Play and Learn with Barney (Blockbuster VHS).6 Time for Camp WannaRunnaRound (Screener).5 Time for It's Time for Counting (2006 VHS).4 Time for Barney In Concert (1995 VHS).3 Time for Let's Go to the Zoo (Screener).2 Time for Barney's Pajama Party (Spanish VHS).1 Time for Camp WannaRunnaRound (Screener & Original). ![]() ![]() ![]()
![]() ![]() Swim hungry" it seems like it doesn't have any time for procreation. I got a letterįrom a guy who did not include his name, but did give me the nickname "Altruismisdead".ĭope fish mates? With its thought patterns just being "swim swim hungry, swim Never really been covered before is how Dopefish reproduce. We got hold of some screen capture software and went to town. Dopefish was myįavorite character, and Steve was the resident Keen expert - Lee provided a few vocalĮffects and some art. Then Steve Quarrella came onboard and it balooned from there. One way to avoid the BugBlatter Beast is to throw a towel over your head.)ĭopefish was "adopted" by the Tech Support staff at Apogee soon after I came onīoard. ![]() It's so stupid that it thinks if you can't see it, itĬan't see you. Heroes." (What's the most dumbest creature in the universe? Why, of course, the His thought patterns go, "swim swim hungry, swim swim hungry."ĭopefish "will eat anything alive and moving near them, though they prefer In the cast of characters for Secret of the Oracle as the second dumbest creature inthe You think, doesn't it? Especially when Tom was no longer with id Software when they The Dopefish was called into being by Tom, it made Tom have a foreshadowing of a future idĪt this cutout from the picture. It was pointed out to me recently that theĭopefish can predict the future. Want to see Tom's original sketches forĭopefish he did in late 1990? Click Here! Stupid little fish," and the rest is history. ![]() Dopefish was one of theĬharacters from these drawings that made the cut. It was one of 24 drawings he did of ideas for charactersįor Commander Keen: Goodbye, Galaxy! while part of id Software. You can read about it here at, the definitive source of info on everyone's favourite fish! All the rest of this "Dopefish mania" came later. What is Dopefish? Dopefish was one of the characters that appeared in Commander Keen, Episode IV back in 1991. ![]() ![]() It’s a great place to find work if you’re just starting out because there is such a wide range of projects available, including copywriting, data entry, customer service, and web development. Upwork is a marketplace that helps match businesses with remote workers for various projects and assignments. And, once you complete a gig, you have the option of paying a $1 fee to receive your funds within 48 hours. ![]() The great thing about Fiverr is that you can set your own schedule and work as much or as little as you want. Although most people think of it as a place to get small tasks done, there are actually many different types of services you can offer on Fiverr, from social media marketing and SEO to resume writing. Fiverrįiverr is a website where you can offer services – or gigs – starting at $5 per gig. Here are some notable freelance hubs that are known for their quick payment times. If you have any other skills that can be done remotely – like web development, graphic design, or SEO – there are dozens of websites where you can find clients and get paid quickly. Writing isn’t the only kind of freelance work that pays out frequently. Listverse is known for paying right away.įor more websites that will pay you to write blog articles, check out this massive list of paid writing gigs! 2. Payment is $100 per article and is made via PayPal. They are currently looking for writers who can contribute well-researched, engaging listicles on any topic they choose. Listverse is a website that publishes lists of 10 items on various topics, ranging from the serious to the whimsical. Just make sure you read their submission guidelines carefully before you send them a pitch! 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With various artistic photo collections and real-time blend modes, you can easily follow your passion for improvising!Ĭombine Photos with Double Exposure Effect and Create Artistic Photos!Īesthetic Photo Merger with Double Exposure Effect!ĭiscover multiple photo blend styles and pre-created previews to create a perfect blend.īlend Photo Editor provides categorized blend effects like Artistic, Space, Galaxy, Nature, City, Mountains, Colorful, Smoky Effect, Texture, Bokeh Light, and more artful photo blends! ![]() ![]() ![]() Nonetheless, we are still in the very early phases of designing artificial proteins for specific functional purposes. Consequently, some groups have steered away from utilizing natural protein scaffolds as a starting point for engineering, and instead create completely artificial, human-made proteins ( Lombardi et al., 2000 Faiella et al., 2009 Anderson et al., 2014 Solomon et al., 2014). Thus, epistatic relationships between residues pose a large challenge to protein engineers who seek to achieve more than a modest improvement in protein activity. For example, the evolutionary trajectory of influenza nucleoprotein revealed that stabilizing substitutions were essential in compensating for otherwise intolerable destabilizing changes that were beneficial to the protein in an adaptive sense ( Gong et al., 2013). Indeed, historical contingency and entrenchment due to epistasis play a critical role in protein evolution ( Weinreich et al., 2006 Bridgham et al., 2009 Bloom et al., 2010 Gong et al., 2013 Harms and Thornton, 2014 Shah et al., 2015). These various historical constraints and complexities can confound engineering approaches. Moreover, natural proteins may also have been subjected to multiple, simultaneous and diverse selection pressures leading to complexity from Darwin's principle of multiple utility ( Dutton and Moser, 2011). Amino acids within natural proteins may display Müllerian interdependency and consequently may have accumulated irreversible complexity ( Dutton and Moser, 2011). Understanding the basis for these constraints has proven challenging, as evolution has sculpted epistatic relationships between residues and structural domains in proteins that are necessary for thermodynamic stability, dynamics, allostery, or complex assembly ( Liberles et al., 2012 Gong and Bloom, 2014 Perica et al., 2014 Sikosek and Chan, 2014). From engineering DNA polymerases to better recognize unnatural nucleotides ( Laos et al., 2014), to introducing catalytic activity into non-enzymatic scaffolds ( Korendovych et al., 2011), protein engineering has enhanced our repertoire of knowledge far beyond solely a fundamental understanding of protein structure and function.ĭespite such progress, there are major challenges to protein engineering, mostly rooted in the abundance of constraints embedded in natural protein sequences and structures ( Dutton and Moser, 2011). A wide spectrum of proteins have been successfully engineered and evolved to adopt new or improved function. An exciting assortment of approaches has been developed to optimize starting protein scaffolds, ranging from the development of site-directed mutagenesis in the early 1980s ( Dalbadie-Mcfarland et al., 1982 Sigal et al., 1982 Winter et al., 1982), to more recent computational design efforts ( Jiang et al., 2008 Siegel et al., 2010). Protein-engineering endeavors have met both challenge and opportunity in designing proteins with desirable or novel functions. The advancement of protein engineering has enriched our understanding of protein structure, function, and folding. They also suggest that enhanced chaperones and disaggregases could have important applications in treating human disease as well as in the purification of valuable proteins in the pharmaceutical sector. Collectively, these advances have revealed key mechanistic and functional insights into chaperone and disaggregase biology. Likewise, small changes in the primary sequence of Hsp104 yield potentiated protein disaggregases that reverse the aggregation and buffer toxicity of various neurodegenerative disease proteins, including α-synuclein, TDP-43, and FUS. Remarkably, enhanced global activity or altered substrate specificity of various molecular chaperones, including GroEL, Hsp70, ClpX, and Spy, can be achieved by minor changes in primary sequence and often a single missense mutation. Here, we review efforts to enhance the activity of individual molecular chaperones or protein disaggregases via engineering and directed evolution. A promising strategy is to engineer proteostasis networks to counter challenges presented by specific diseases or specific proteins. Nevertheless, proteostasis networks have limits, which when exceeded can have fatal consequences as in various neurodegenerative disorders, including Parkinson's disease and amyotrophic lateral sclerosis. ![]() Critical components of this network include molecular chaperones and protein disaggregases, which function to prevent and reverse deleterious protein misfolding. 2Biochemistry and Molecular Biophysics Graduate Group, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA, USAĬells have evolved a sophisticated proteostasis network to ensure that proteins acquire and retain their native structure and function.1Department of Biochemistry and Biophysics, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA, USA. ![]() ![]() ![]() With Starfield being the center of the Xbox 2023 Showcase last week, Bethesda gave us a deep dive into one of the biggest games this generation. Is it ok to shout "Believe it!" right now? No? Alright. Be sure to check out this awesome monster of an interivew, and keep looking to IGN.com for more exclusive Naruto action. We had a chance to sit down and chat with the living stars of the show to talk about the Naruto anime, Clash of Ninja, and the rabid fan-base it enables. Could a little Naruto swag be on the way for a few lucky IGN readers? It's very possible! But wait, there's more! As we were busying ourselves with tasty chicken and four player mayhem, Naruto voice actors Tara Platt (voice of Temari), and Yuri Lowenthal (voice of Sasuke) crashed the party. Jealous yet? That's alright, as our good friend Kirk Green (responsible for making sure IGN can give you all the first look at Naruto media, builds, and info) eluded at the possibility of a fan contest in the near future. The bag-o-goodies really is impressive, as it includes a Naruto laptop bag, keychain, shoulder bag (which is huge as well), collectable mini-tins, Volume 1 of the TV show, trading cards and a leaf headband (which is by far the most popular piece of swag at this year's show… seriously). Life is hilariously cruel… We check out some of our sweet swag back at the hotel. Not in the sense that you'll be getting any of the kick-ass swag… just meaning you can look at all the cool stuff that we get and you don't. The collection was so impressive, we just had to share it with all of you. To top the evening off, D3 also treated members of the press to one of the best swag-bags we've seen in a long time. The Red Pearl restaurant was also topped off with volumes of the Naruto manga, so we had a chance to catch up on a little reading while waiting our turn to kick some ninja butt. The character-swapping ability has been added to an otherwise simple beat-em-up adventure game, speckled with new technical additions like swinging platforms and parallax presentation for backgrounds. On the GBA side of things, Naruto: Ninja Council 2 held up pretty well for being an Americanized version of a four year old game, but was a bit on the basic side. two fights, and even a few deadly 3-on-1 bouts. ![]() During the event we had a chance to sharpen our skills once again in free-for-all battles, two vs. For those not familiar with the game, Clash of Ninja 2 allows for four-player simultaneous action, and there are a ton of options to go with it. Since we've had a chance to go hands-on with Naruto 2 once before, the IGN crew already had a healthy advantage over the other contenders, and needless to say, we made quick work of them. Well there were, and they were pretty fun too. Wait… did we mention there were games there? Whoever thought tasting stuff would be so good? But enough about amazing chicken wings that taste so sweet as to be called the nectar of the gods, we've got gameplay to get to. Even though the combination of GameCube controllers and glazed wings is often frowned upon at " La Casa de Casamassina", publisher D3 had no fear, and for that reason, the party rocked. Aside from top-tier grub and restaurant-exclusive "Naruto Cocktail", we had the chance to go hands-on with Clash of Ninja 2 in some serious four-player competition, as well as run through practically all of Ninja Council 2. The party took place at local hotspot "The Red Pearl," which offered not only an awesome atmosphere, but some of the best teriyaki chicken wings we've ever had (what can we say… they rocked). Since all work and no play makes IGN a fussy baby, we decided to head over to the "Naruto Preview Party", which showcased Clash of Ninja 2 (GCN), Naruto: Ninja Council 2 (GBA), and a crap-load of awesome Japanese cuisine. If not for anything else, Comic-Con can be counted on for two things: Sweaty nerds and rockin' parties. ![]() |
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